Effectivity of entomopathogenic fungus Beauveria bassiana to control white grub Lepidiota sp.

Brotodjojo, R.R. Rukmowati and Solichah, Chimayatus (2011) Effectivity of entomopathogenic fungus Beauveria bassiana to control white grub Lepidiota sp. In: The 15th International Conference for Women Engineers and Scientists, 19-22 July 2011, Adelaide, Australia.

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Abstract

Entomopathogenic fungus Beauveria bassiana is soil inhabited fungus that known to have a wide range of hosts. Previous work showed that this fungus can be cultured in solid media using rice bran. The objective of this research was to study the effectivity of B. bassiana cultured in rice bran to control Lepidiota sp. in semi field experiment. Two grubs were inoculated into seven-weeks old soybean planted in a polybag. The fungus was applied one week after grub inoculation, in three levels of dose, i.e. 1 g cultured fungus/100 g soil; 1.5 g cultured fungus/100 g soil; 2 g cultured fungus/100 g soil, and control with no fungal application. The applications of the fungus were conducted with two methods, first, the solid cultured fungus was applied directly into soil around root area, and second, the solid cultured fungus was diluted into 400 mL water and the fungal solution was poured on the soil. The results showed that the mortality of treated grubs was not significantly different from those untreated grubs. However, the weight of fresh root, fresh plant and dry plant of plant treated directly with 1.5 g cultured fungus/100 g soil was significantly higher than those of other treated plants and untreated plant. The direct application of 1.5 g cultured fungus/100 g soil resulted in higher number of seed per plant than other applications. In conclusion, this fungus can be used to control Lepidiota sp., although higher dose may be needed. Key words: Effectivity, Entomopathogenic Fungus, Beauveria bassiana, White Grub, Lepidiota sp

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