INDUCING SOMATIC EMBRYOS OF SOYBEAN GLYCINE MAX AND GLYCINE SOJA ON SUCROSE CONCENTRATIONS VARIATION

WAHYURINI, ENDAH (2011) INDUCING SOMATIC EMBRYOS OF SOYBEAN GLYCINE MAX AND GLYCINE SOJA ON SUCROSE CONCENTRATIONS VARIATION. International Conference on Sustainable Agriculture and Food Security: Chal lenges and Opportunities, - (-). pp. 445-451. ISSN 978-979-8246

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Abstract

Production and productivity of Soybean in Indonesia have not met d nationol demand. The government progrom is to improve Soybeon productivity ond production to ochieve self sufficiency on 2015. Genotype ond sucrose in the medium tissue culture is importont inducing somqtic embryos. The objectives of this experiment wds to inducing somatic embryos of Glycine mox vorieties Anjosmoro and Glycine soja vorieties Mallika at the voriation of sucrose concentration for MS medium by in vitro. The reseqrch was used immoture cotyledons explant which wos conducted at Greenhouse ond Biotechnology Laboratory, Agrotechnology of UPN "Veteron" Yogyokorto, lndonesia from Mei to October 2010. The experiments were arronged in factorial Completely Randomized Design with two foctors ond replicated ten times. The first foctor was explonts soybeons were: Mallika (Glycine soja) ond Anjosmoro (Glicyne mox). The second foctor was concentration sucrose: 20 gfl. 30 g/, ond 40 7fl. Doto were subjected to an anolysis of varionce followed by Dunnet's Significonce Test (DST) ot 5% significance level. The results showed that moterial explant Glycine mox and Glicyne soja not induced somotic embryos. The best sucrose concentrotions 20 gl for MS medium increased to time of embryos (doys), growth percentage of embryos ond fresh weight callus. The combinotion treatment Glicyne max ond sucrose 20 ql to increosed dry weight callus. Keyword: Embryos somatic, soybean, sucrose

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