The Influence of PH and Ionic Strength on The Concentration of -Amylase Enzyme Using Electrofiltration

Permadi, Adi and I gede, Wenten The Influence of PH and Ionic Strength on The Concentration of -Amylase Enzyme Using Electrofiltration. The Influence of PH and Ionic Strength on The Concentration of -Amylase Enzyme Using Electrofiltration. (Submitted)

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    Abstract

    Enzyme is one of the important materials in the bioprocess industry. In order to get an enzyme as a product, a suitable separatory unit is required in the production process. This is because enzyme can be easily deactivated by heat, chemicals and physical treatments as well as hydrolyzed by other enzymes. Various techniques can be used in the separatory process, among others electrophoresis, chromatography, adsorption, crystallization, centrifugation and ultrafiltration. This research tried to investigate the influence of the pH at different values of 5,3; 7,1 and 9,1 also ionic strength using NaCl 5% w/v at a concentrated α–amylase using electrofiltration. The study was performed using an enzyme solution as a feed and water as a diluate into electrofiltration membrane that were connected to 60 volt power supply. From the experiment, it was found that the selectivity of water towards the enzyme’s components increased with an increase in the concentration level (VCR). The highest selectivity; 31.27 was obtained at an eznyme solution with pH 5.3 with salt addition (5 % w/v NaCl). The highest selectiviy obtained in the absence of salt was 14.62 at pH 7.1. An increase in a specific activity generally occured at 2 times concentration further decreased at 5 time concentration for an enzyme solution using NaCl ( 5% w/v). The largest % rejection obtained was 71.12 at 5 times concentration of enzymatic solution with salt addition at pH 5.3. Keywords: Amylase, ionic strength, pH, electrofiltration, selectivity.

    Item Type: Article
    Subjects: 600 Teknologi
    Divisions: UNSPECIFIED
    Depositing User: Wikan Pinandhito
    Date Deposited: 13 Jul 2011 11:14
    Last Modified: 13 Jul 2011 11:14
    URI: http://repository.upnyk.ac.id/id/eprint/563

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